Technician - Hubrecht Institute - Utrecht
Location: Utrecht
Max hours per week: 38,00
Max salary: €3.636
Duration of agreement: 1 year (extension after positive evaluation)
Apply until: 01/15/2024
The Sahtoe lab at the Hubrecht Institute is looking for a full time highly motivated research technician for one year with possibility for extension based on merit. The Sahtoe group uses protein design approaches to create new protein tools for synthetic biology applications and to understand the fundamental biology of epigenetics and allosteric protein regulation.
As a key member of the lab, you will maintain and streamline the organization of the lab. In addition, you will perform wet lab experiments in relation to expression, purification and in vitro characterization of computationally designed proteins. You will use techniques such as recombinant protein expression in E.coli, molecular cloning, immobilized metal affinity chromatography (IMAC), size exclusion chromatography (SEC) and biolayer interferometry (BLI) to measure the interactions between biomolecules and will work closely together in a team with PhD students and Postdocs. Depending on (programming) skills and interests, there is also the possibility to learn how to design novel proteins under supervision of more experienced lab members.
Requirements:
You have completed a HLO (or equivalent) program, or a MSc program in Life Sciences or related sciences, and are proficient in English. Strong social/communication skills and a collaborative team spirit are vital. Experience with recombinant protein expression in E.coli, cloning, IMAC, SEC and BLI is a pre but is not absolutely required. However, willingness to learn and master these techniques is required. Experience with mammalian cell culture and/or basic programming/bioinformatics is not required but would be advantageous.
About the Hubrecht Institute
The Hubrecht Institute is a research institute focusing on developmental and stem cell biology. At the institute, more than 20 research groups perform fundamental and multidisciplinary research, both in healthy systems and disease models. The Hubrecht Institute is a research institute of the Royal Netherlands Academy of Arts and Sciences (KNAW) and is located on the Utrecht Science Park ‘De Uithof’. Since 2008, the institute has been affiliated with the University Medical Center Utrecht, advancing the translation of research into clinical practice. The Hubrecht Institute has a partnership with the European Molecular Biology Laboratory (EMBL).
Terms of employment
Depending on education and experience the minimum salary is €2.693 and the maximum salary is €3.636 gross per month for a full-time appointment (scale 7 cao Nederlandse Universiteiten/KNAW). This is exclusive of 8% vacation allowance, 8.3% year-end bonus, travel allowance, internet allowance, home working allowance and pension accrual with ABP.
The KNAW offers its staff an excellent package of secondary benefits. A package that meets the different needs of employees depending on their stage of life, lifestyle or career ambitions. For example, by working an extra two hours a week, it is possible to increase the number of days off from 29 to 41 days a year (with full-time employment).
For a complete overview of the terms of employment, please refer to the web page: werken bij de KNAW.
Diversity & Inclusion
The KNAW considers a working environment in which everyone feels welcome and appreciated of great importance. A working environment in which attention is paid to individual quality and where development opportunities are paramount. Together we strive for an inclusive culture in which we embrace differences. We would therefore like to invite candidates who want to contribute to this through their background and experience. In the event of equal suitability, preference will be given to the candidate who thus enhances diversity within the Academy.
We will not respond to any supplier enquiries based on this job advertisement.